2019 session 1 ================ FVB/N Mice Spontaneously Heal Ulcerative Lesions Induced... ----------------------------------------------------------- Means +/- SD are represented in the histograms. *p < 0.05, **p < 0.01 (Mann-Whitney U test). Bars represent the mean value. *p < 0.05 (Wilcoxon test). Means +/- SD were plotted. Nodes of FVB/N were analyzed (n = 5 mice for each time point) No statistical analysis section. Maternal obesity impairs fetal cardiomyocyte contractile function in sheep -------------------------------------------------------------------------- Statistical analysis Prism software (GraphPad, La Jolla, CA, USA) was used for statistical analysis. Results are expressed as means 6 SEM. Statistical significance was determined with an unpaired 2-sided t test analysis of differences between the 2 specified groups: control andMO. Significance was set at values of P < 0.05. LV weight (n = 5 control fetuses and n = 4 MO fetuses). LV thickness (n = 7 control fetuses and n = 7 MO fetuses). Means +/- SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control. The urea decomposition product cyanate promotes endothelial dysfunction ------------------------------------------------------------------------ Data are expressed as mean±s.e.m. (n=3-4). Statistical analysis was performed by one-way analysis of variance (ANOVA) for more than two groups and with Student's t-test for two groups. Significance was accepted at *P<0.05, **P<0.01 vs. control. Statistical analyses Data are shown as mean±s.e.m. for n observations with the exception of EC50 values (ACh concentrations required to achieve 50% of maximal relaxation), which were expressed as mean with 95% confidence intervals. Comparison of multiple groups was performed using one-way analysis if variance with Tukey's multiplecomparison post hoc test (HCAEC experiments). Student's t-test was used to test for differences between control and cyanate-treated mice, whereas for vascular function studies two-way analysis of variance for repeated measurements followed by a Bonferroni's post hoc test was used. Significance was accepted at Po0.05. Statistical analyses were performed with Prism Version 4.03 (GraphPad Software, La Jolla, CA). Profiling the relationship between tumor-associated macrophages and pharmacokinetics ------------------------------------------------------------------------------------ of liposomal agents in preclinical murine models ----------------------------------------------- Statistical analysis. Statistical analyses were carried out using Prism for Windows software v3.03 (GraphPad Software, Inc.). Significance comparing H-scores and comparing AUC0-96h within plasma and tissues were calculated using two group t-test. The Kaplan-Meier (KM) method was used to estimate survival curves and median survival, along with 95% confidence intervals, and the log-rank test was used to compare the overall survival between groups for the efficacy studies. A P-value of less than 0.05 was considered statistically significant. All statistical tests were two-sided, and unadjusted p-values are reported. A significant difference was observed between breast cancer cell lines and those of ovarian and endometrial cancers within whole tumor samples (P=0.023 & 0.036, respectively). P-values were calculated using two-sided unpaired t-test. Statistical significance was defined as a p-value < 0.05 - 0.01 (*) or < 0.01 - 0.001 (**). Profile of French general practitioners providing ------------------------------------------------- opportunistic primary preventive care ------------------------------------- Values are median [minimum-maximum] or number (percentage). Statistical analyses The endpoint of the present study was to describe the GP's proportion of preventive consultations. Categorical variables were described using number and percentage. Continuous variables were described using median and [minimum-maximum]. The aggregate data per GP were reported as median and [minimum- maximum] GP's proportion. Using a univariate linear regression, we assessed the relationship of the proportion of preventive consultations per GP with his/her personal characteristics, the sociodemographic characteristics of his/her patients and his/her consultation context. The multivariate linear regression analysis included all the variables with a P value <=0.20 based on the aforementioned univariate analysis. A backward selection on P value was performed to retain the significant variables. We performed all data analyses using R-software, version 3.0.2 (R Foundation, from http:// www.r-project.org, Auckland, New Zealand). GPs performed more preventive consultations if they were practising in an urban rather than a rural location (+9.78, SE = 3.31, P = 0.004) or in Southeast France rather than in the Paris metropolitan area (+8.17, SE = 4.07, P = 0.047). Assessing in vitro combinations of antifungal drugs against ----------------------------------------------------------- yeasts and filamentous fungi ---------------------------- Data obtained as described above were analyzed using four different models, used previously to analyze antifungal drug interactions [8,17,18]. The models were parametric and non-parametric approaches of the following two zero-interaction theories: Loewe additivity and Bliss independence.I Loewe additivity (LA) is described by the following equation [...] where dA and dB are the concentrations of the drugs A and B in the combination that elicits a certain effect, and DA and DB the iso-effective concentrations of the drugs A and B when acting alone. FIC index model The non-parametric approach is based on the fractional inhibitory concentration index model expressed with the following equation [...] This model was fitted directly to all experimental data of a data set (percentages of growth for all concentrations of the two drugs alone or in combination) with a non-weighted, non-linear regression analysis using the MODLAB program (MEDIMATICS, Maastricht, The Netherlands, www.medimatics.nl). When the estimate of a was positive and its 95% confidence interval (95% CI) did not include 0, statistically significant synergy was claimed, while when a was negative without its 95% CI overlapping 0, statistically significant antagonism was concluded. In any other case Loewe additivity/ indifference was concluded. Goodness of fit criteria included the 95% CI of the estimated parameters, the R2, the sum of the squares (SumSq), correlation and covariance matrices. In order to check for any systematic deviation of the model from the data, residual plots were constructed and deviation from the normal distribution was tested with the Kolmogorof/Smirnof (KS) test. COMPLEXE XYLOPHAGE ASSOCIÉ AU DÉPÉRISSEMENT DU CÈDRE DE L'ATLAS AU BÉLEZMA ------------------------------------------------------------------------------- Durant cette étude 1306 individus d'insectes xylophages ont été récoltés (Tab. 1) par deux méthodes de piégeage et ceci pendant 12 mois de suivi. Pas de rubrique Analyse Statistique. Aucun calcul statistique. Musculoskeletal Disorders: Prevalence and Associated Factors ------------------------------------------------------------ among District Hospital Nurses in Haiphong ------------------------------------------ 2.4. Statistical Analysis. The data was imported and analyzed using SPSS 19.0 version software. We used algorithms for descriptive statistics (percentage, mean, median, standard deviation, and so forth) to describe the sociological characteristics of the participants. The prevalence of MSDs was calculated as the percentage of nurses who developed symptoms of MSDs in at least one of the nine positions on the body (showed in the Nordic Questionnaire). We used chi-squared test when comparing two percentages and t-test when comparing two means. Multiple logistic regressions were used to analyze and identify factors associated with MSDs. The models were checked for fitness using Hosmer- Lemeshow's goodness-of-fit test. All analyses were carried out in the Faculty of Public Health, Haiphong University of Medicine and Pharmacy.The level of significance was set at a p-value of less than 0.05. Mycobacterial Toxin Induces Analgesia in Buruli Ulcer by Targeting the Angiotensin Pathways ------------------------------------------------------------------------------------------- (n = 5 redness stage mice, n = 5 edema mice). Mean ± SD, ***p < 0.0001, Student's t test. (D) Quantification of the fluorescence intensity expressed as the ratio of bis-oxonol (DiSBAC2(3)) signal intensity at 20 min (T2int) to that at 0 min (T1int). Mean ± SD. (E and F) Effect of two K+ channels blockers, tetraethylammonium (TEA) and barium chloride (BaCl2) on the cell hyperpolarization caused by mycolactone. Mean ± SEM. No statistical analysis section. Increased Total mtDNA Copy Number --------------------------------- Data are represented as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001. QUANTIFICATION AND STATISTICAL ANALYSIS Statistical analysis (except proteomics data) was conducted using GraphPad Prism 5 software. For all experiments where the N > 6, data were assessed for normality using Shapiro-Wilk test. For all other experiments the data were assumed to follow a normal distribution. All data are presented as means ± SEM. Statistical comparisons between two groups were performed using Student's t test; comparisons between more than two groups were performed using one-way analysis of variance (ANOVA), post hoc analysis was conducted with Tukey's multiple comparison test. Values of p < 0.05 were considered statistically significant. All statistical parameters are outlined in the relevant figure legends. For proteomics, analysis was conducted using Perseus statistical framework version 1.5.2.4. Multi-sample comparison (ANOVA analysis, Benjamini-Hochberg FDR 0.05 was used for significant truncation) was performed. Critical Hydrogen Bond Formation for Activation of the Angiotensin II Type 1 Receptor -------------------------------------------------------------------------------------------- Analysis of the 1 and 2 angles (x and y axes, respectively) of the side chain of residue D742.50 (A) and N461.50 (B) from 2100 frames of the 84-ns MD simulation of the AT1 receptor (blue), N111G-AT1 receptor (red) and N111W-AT1 receptor (green). Colored squares represent the average 1 and 2 values in each simulation. 84-ns MD simulation of the AT1 receptor (blue), N111G-AT1 receptor (red) and N111W-AT1 receptor (green). Colored squares represent the average 1 and 2 values in each simulation. On average, the distance between these two atoms was 9.1 0.5 Å for the AT1 receptor and Data are the mean +/- S.D. (error bars) of 2100 frames from each 84-ns MD trajectory. *, significantly different (p < 0.01). No statistical analysis section. The Role of a Sodium Ion Binding Site in the Allosteric Modulation of... -------------------------------------------------------------------------------------------- The root-mean-square deviation (rmsd) of the ion with respect to its crystallographic position is indicated with a black line, while the horizontal bar at 1.8A° (the resolution of the parent crystal structure) denotes the limit for the crystallographic coordination state (position c1). The error bars indicate the SD estimated from three MD replicas (n = 3); ami, amiloride; ZM, ZM241385. Significantly different from the control simulation (i.e., absence of any allosteric ligand) in a Student's t test with *p < 0.05. Values are means ± SEM of two to five separate assays performed in duplicate. Data Analysis The radioligand binding and thermostability data were processed with Prism 5 (GraphPad). Statistical significance was assessed with a Student's t test. Tracking the best reference genes for RT-qPCR data normalization in filamentous fungi ------------------------------------------------------------------------------------- Gene expression and statistical analysis [...] Statistics (mean ± SD) were assessed from FC values obtained from biological replicates. Other statistical analyses were conducted by using the STATGRAPHICS Centurion 16 software. This included: the ANOVA on relative FC values (Three-level, nested ANOVA with 'genes' as the first level, 'culture conditions' as the second level and 'biological replicates' as the third level); the Hierarchical Ascendant Classification (HAC) that was performed according to the Ward method, using default parameters (standardization of the data and squared Euclidean distances); the graphical representation of box plots. The relaxant effect of the Montivipera bornmuelleri snake venom ----------------------------------------------------------------- The maximum effect (Emax) obtained for the highest concentration (50\u03bcg/ml) is 98.22%±4.232 [...] The relaxation percentage obtained for the highest concentration of venom were, respectively, 92.97%±2.360 (n=13), 87.132%±4.07 (n=8), 81.150%±5.252 (n=10), 99.99%±0.006 (n=6), 84.937±5.021% (n=13). [...]with an Emax of 0,917gm±0.081 with a venom concentration of 5×10-6gm/ml (n=6, p<0.0001 vs. control), 1,323g±0.36 with a venom concentration of 5×10-5gm/ml (n=6, p<0.0001 vs. control) and 0,203gm±0.053 for nicardipine (n=6, p<0.0001 vs control). No statistical analysis section. Impact of nutrient excess and endothelial nitric oxide synthase on the plasma metabolite profile in mice -------------------------------------------------------------------------------------------------------- STATISTICAL ANALYSES Data are presented as mean ± s.e.m. Multiple groups were com- pared using One-WayANOVA, followed by Bonferronipost-tests. Unpaired Student's t-test was used for directcomparisons. For statistical analyses the Metaboanalystand/or GraphPad5.0 soft- ware was used. A p <= 0.05 was considered significant. lean mass percentage following 6 weeks of diet measured by Dexascan analysis. n = 7 pergroup; p < 0.001 vs. indicated group; #p < 0.05, ##p < 0.01,and ###p < 0.001vs. WT HFD. One-Way ANOVA n = 28, with 7 mice per group. CO2 sensing in fungi: at the heart of metabolic signalingd ---------------------------------------------------------- No stats. No statistical analysis section.